Volume 16, Issue 5 (5-2018)                   IJRM 2018, 16(5): 335-340 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Barati S, Movahedin M, Batooli H. In vitro antiapoptotic effects of the calligonum extract on spermatogonial stem cells. IJRM. 2018; 16 (5) :335-340
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1109-en.html
1- Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , movahed.m@modares.ac.ir
3- Kashan Research Station (Kashan Botanical Garden), Research Division of Natural Resources, Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Isfahan, Iran
Abstract:   (41 Views)
Background: Spermatogonial stem cells are the foundation of spermatogenesis and male fertility. So, their maintenance and culture are very important.
Objective: In this study, we assessed protective effects of the Calligonum on in vitro viability and apoptotic and antiapoptotic genes expression of spermatogonial stem cells.
Materials and Methods: After 24 hr of culture, the spermatogonial stem cells were treated with 30 μM dose of H2O2 and then 10 μg/ml the Calligonum extract was added for 3 wks. Viability was assessed by Trypan blue, apoptosis using PI-Annexin and finally Bax, Bcl-2 and P53 genes expression by Real-Time Polymerase chain reaction.
Results: After 3 wk of treatment, viability in the Calligonum extract+H2O2 group was significantly higher than H2O2 group alone (p=0.001). In the Calligonum extract+H2O2 group, apoptosis, as well as expression of apoptotic genes (Bax and P53), was significantly lower than the group treated with H2O2 alone.
Conclusion: The results of this study showed that 30 μM H2O2 increased apoptosis but decreased viability in spermatogonial stem cells. Calligonum has antioxidant properties that can reduce apoptosis, Bax and P53 expression and increase the viability and Bcl-2 expression.
Full-Text [PDF 392 kb]   (30 Downloads) |   |   Full-Text (HTML)  (6 Views)  
Type of Study: Original Article |
Received: 2018/06/26 | Accepted: 2018/06/26 | Published: 2018/06/26

1. Niu Z, Goodyear SM, Avarbock MR, Brinster RL. Chemokine (C-X-C) ligand 12 facilitates trafficking of donor spermatogonial stem cells. Stem Cells Int 2016; 2016: 5796305. [DOI:10.1155/2016/5796305]
2. Morena AR, Boitani C, Pesce M, De Felici M, Stefanini M. Isolation of highly purified type A spermatogonia from prepubertal rat testis. J Androl 1996; 17: 708-717.
3. Huleihel M, Lunenfeld E. Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl 2004; 6: 259-268.
4. Alvarez JG, Touchstone JC, Blasco L, Storey BT. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. J Androl 1987; 8: 338-348. [DOI:10.1002/j.1939-4640.1987.tb00973.x]
5. Chaudière J, Ferrari-Iliou R. Intracellular antioxidants: from chemical to biochemical mechanisms. Food Chem Toxicol 1999; 37: 949-962. [DOI:10.1016/S0278-6915(99)00090-3]
6. Szeto HH. Cell-permeable, mitochondrial-targeted, peptide antioxidants. AAPS J 2006; 8: E277-283. [DOI:10.1007/BF02854898]
7. Singh M, Sharma H, Singh N. Hydrogen peroxide induces apoptosis in HeLa cells through mitochondrial pathway. Mitochondrion 2007; 7: 367-373. [DOI:10.1016/j.mito.2007.07.003]
8. Agarwal A, Gupta S, Sikka S. The role of freeradicals and antioxidants in reproduction. Curr Opin Obstet Gynecol 2006; 18: 325-332. [DOI:10.1097/01.gco.0000193003.58158.4e]
9. Nagata H, Takekoshi S, Takagi T, Honma T, Watanabe K. Antioxidative action of flavonoids, quercetin and catechin, mediated by the activation of glutathione peroxidase. Tokai J Exp Clin Med 1999; 24: 1-11.
10. Ha SJ, Kim BG, Lee YA, Kim YH, Kim BJ, Jung SE, et al. Effect of antioxidants and apoptosis inhibitors on cryopreservation of murine germ cells enriched for spermatogonial stem cells. PloS One 2016; 11: e0161372. [DOI:10.1371/journal.pone.0161372]
11. Hashemi E, Akhavan O, Shamsara M, Daliri M, Dashtizad M, Farmany A. Synthesis and cyto-genotoxicity evaluation of graphene on mice spermatogonial stem cells. Colloids Surf Biointerfaces 2016; 146: 770-776. [DOI:10.1016/j.colsurfb.2016.07.019]
12. Shi L, Zhao H, Ren Y, Yao X, Song R, Yue W. Effects of different levels of dietary selenium on the proliferation of spermatogonial stem cells and antioxidant status in testis of roosters. Anim Reprod Sci 2014; 149: 266-272. [DOI:10.1016/j.anireprosci.2014.07.011]
13. Batooli H, Haghir-Ebrahimabadi A, Choromzadeh M, Hadizade Hafshejani M, Mazoochi A. The survey and comparison of the essential oils chemical composition of two Calligonum L. species from Aran & Bidgol area. Eco-phytochem J Med Plants 2013; 1: 13-24.
14. Jahromi MA, Movahedin M, Amanloo M, Mowla JS, Mazaheri Z, Batouli H. [The effects of calligonum extract on sperm parameters and the rate of apoptosis in aged male mice testis issue.] Modares J Med Sci Pathobiol 2013; 16: 41-54. (in Persian)
15. Badria FA, Ameen M, Akl MR. Evaluation of cytotoxic compounds from calligonum comosum L growing in Egypt. Z Naturforsch C 2007; 62: 656-660. [DOI:10.1515/znc-2007-9-1005]
16. Boonsorn T, Kongbuntad W, Narrkong N, Aengwanich X. Effects of catechin addition to extender on sperm quality and lipid proxidation in boar semen. Am Eur J Agric Environ Sci 2010; 7: 283-288.
17. Shalabi M, Khilo K, Zakaria MM, Elsebaei MG, Abdo W, Awadin W. Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells. Asian Pac J Trop Biomed 2015; 5: 375-381. [DOI:10.1016/S2221-1691(15)30372-5]
18. Barati S, Movahedin M, Mazaheri Z, Batooli H, Tahmasebi F. Establishment of oxidative stress modeling during spermatogonial stem cells cultivation treated with different doses of H2O2. Anat Sci J 2015; 12: 23-28.
19. Fakhraei M, Nejati V, Delirazh N. Vanadium Schiff base compound mediated apoptosis and cytotoxicity effects in K562 cell line. J Army Univ Med Sci 2012; 10: 185-191.
20. Honaramooz A, Megee SO, Dobrinski I. Germ cell transplantation in pigs. Biol Reprod 2002; 66: 21-28. [DOI:10.1095/biolreprod66.1.21]
21. Ogawa T. Spermatogonial transplantation: the principle and possible applications. J Mol Med 2001; 79: 368-374. [DOI:10.1007/s001090100228]
22. Oatley JM, Avarbock MR, Telaranta AI, Fearon DT, Brinster RL. Identifying genes important for spermatogonial stem cell self-renewal and survival. Proc Natl Acad Sci USA 2006; 103: 9524-9529. [DOI:10.1073/pnas.0603332103]
23. Brinster RL, Zimmermann JW. Spermatogenesis following male germ-cell transplantation. Proc Natl Acad Sci USA 1994; 91: 11298-11302. [DOI:10.1073/pnas.91.24.11298]
24. Zhou DX, Qiu SD, Zhang J, Tian H, Wang HX. The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. Asian J Androl 2006; 8: 584-588. [DOI:10.1111/j.1745-7262.2006.00198.x]
25. Halliwell B. Cell culture, oxidative stress, and antioxidants: avoiding pitfalls. Biomed J 2014; 37: 99-105. [DOI:10.4103/2319-4170.128725]
26. Weisiger RA, Fridovich I. Mitochondrial superoxide dismutase Site of synthesis and intramitochondrial localization. J Biol Chem 1973; 248: 4793-4796.
27. Sikka SC. Oxidative stress and role of antioxidants in normal and abnormal sperm function. Front Biosci 1996; 1: e78-86. [DOI:10.2741/A146]
28. Lai HC, Yeh YC, Wang LC, Ting CT, Lee WL, Wang KY, et al. Propofol ameliorates doxorubicin-induced oxidative stress and cellular apoptosis in rat cardiomyocytes. Toxicol Appl Pharmacol 2011; 257:437-448. [DOI:10.1016/j.taap.2011.10.001]
29. Rengarajan T, Nandakumar N, Rajendran P, Haribabu L, Nishigaki I, Balasubramanian MP. D-Pinitol Promotes Apoptosis in MCF-7 Cells via Induction of p53 and Bax and Inhibition of Bcl-2 and NF-kB. Asian Pac J Cancer Prev 2014; 15: 1757-1762. [DOI:10.7314/APJCP.2014.15.4.1757]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2018 All Rights Reserved | International Journal of Reproductive Biomedicine

Designed & Developed by : Yektaweb