Volume 16, Issue 5 (May 2018)                   IJRM 2018, 16(5): 315-322 | Back to browse issues page

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Kheirollahi A, Abbaszadeh A, Anbari K, Rostami B, Ahangari A, Hasanvand A et al . Troxerutin protect sperm, seminiferous epithelium and pituitary-gonadal axis from torsion-detorsion injury: An experimental study. IJRM. 2018; 16 (5) :315-322
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1107-en.html
1- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran , rezagholami57@gmail.com
2- Department of Surgery, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
3- Social Determinant of Health Research Center, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4- Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
5- Department of Anatomical Sciences, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract:   (394 Views)
Background: Troxerutin is a flavonoid antioxidant that protect different organ against damage caused by ischemia-reperfusion.
Objective: The aim of this study was to evaluate the effect of troxerutin in reducing the damages caused by ischemia-reperfusion in rat's testis.
Materials and Methods: 40 Male Wistar rats (2 month old) were divide to four groups (n=10). Group1 (sham), Group 2 (control, ischemia-reperfusion (I/R) without treatment), Group 3 (I/R+150 mg/kg of troxerutin), and group 4 (I/R+20 mg/kg of vitamin C). Treatment of group 3 and group 4 during torsion (twists 720 counter clock at 90 min) followed by 50 days detorsion. After 50 days, blood samples were collected and rats in all study groups were killed and their testes were removed, and fixed with Bouin’s solution. Testis was stained with hematoxylin and eosin dye and the level of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured with ELISEA methods. TUNEL was employed to detect apoptosis. Epididymis caudal part was removed and total sperm count was determined. Johnson techniques were used for assessment of seminiferous tubules quality.
Results: Troxerutin treated group has higher Johnson score’s (p≤0.001), antiapoptotic properties (p≤0.001), sperm count (p=0.065), and higher LH (p≤0.001), FSH (p≤0.001) and testosterone (p=0.002) levels than control group. Vitamin C treated group showed increase level of testosterone but didn’t show significant differences on the number of apoptotic cells, Johnson scores, LH, FSH and sperm count than control group.
Conclusion: Troxerutin has protective effects on testicular torsion induced injury and can ameliorate spermatogenesis in the torsion-detorsion models.
Full-Text [PDF 996 kb]   (176 Downloads) |   |   Full-Text (HTML)  (63 Views)  
Type of Study: Original Article |
Received: 2018/06/26 | Accepted: 2018/06/26 | Published: 2018/06/26

1. Agarwal A, Allamaneni SS. Free radicals and male reproduction. J Indian Med Assoc 2011; 109: 184-187.
2. Gholami M, Abbaszadeh A, Khanipour Khayat Z, Anbari K, Baharvand P, Gharravi AM. Honey improves spermatogenesis and hormone secretion in testicular ischaemia-reperfusion-induced injury in rats. Andrologia 2018; 50: 1-5. [DOI:10.1111/and.12804]
3. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. world J Mens Health 2014; 32: 1-17. [DOI:10.5534/wjmh.2014.32.1.1]
4. Doshi SB, Khullar K, Sharma RK, Agarwal A. Role of reactive nitrogen species in male infertility. Reprod Biol Endocrinol 2012; 10: 109. [DOI:10.1186/1477-7827-10-109]
5. Agarwal A, Allamaneni SS. Free radicals and male reproduction. J Indian Med Assoc 2011; 109: 184-187.
6. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002; 29: 817-827. [DOI:10.1016/S0094-0143(02)00081-2]
7. Aitken RJ, Clarkson JS, Fishel S. Generation of reactive oxygen species, lipid peroxidation, and human sperm function. Biol Reprod 1989; 41: 183-197. [DOI:10.1095/biolreprod41.1.183]
8. Billig H, Furuta I, Rivier C, Tapanainen J, Parvinen M, Hsueh A. Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stages. Endocrinology 1995; 136: 5-12. [DOI:10.1210/endo.136.1.7828558]
9. Ikeda M, Kodama H, Fukuda J, Shimizu Y, Murata M, Kumagai J, et al. Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biol Reprod 1999; 61: 393-399. [DOI:10.1095/biolreprod61.2.393]
10. Showell MG, Brown J, Yazdani A, Stankiewicz MT, Hart RJ. Antioxidants for male subfertility. Cochrane Database Syst Rev 2011; 1: 1-7. [DOI:10.1002/14651858.CD007411.pub2]
11. Durairajanayagam D, Agarwal A, Ong C, Prashast P. Lycopene and male infertility. Asian J Androl 2014; 16: 420-425. [DOI:10.4103/1008-682X.126384]
12. Gholami M, Saki G, Hemadi M, Khodadadi A, Mohammadi-Asl J. Melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice. Iran J Basic Med Sci 2014; 17: 93-99.
13. Gholami M, Saki G, Hemadi M, Khodadadi A, Mohamma-di-Asl J. Effect of Melatonin on the Expression of Apoptotic Genes in Vitrified-thawed Spermatogonia Stem Cells Type A of 6-Day-Old Mice. Iran J Basic Med Sci 2013; 16: 906-909.
14. Gholami M, Khayat ZK, Anbari K, Obidavi Z, Varzi A, Boroujeni MB et al. Quercetin ameliorates peripheral nerve ischemia-reperfusion injury through the NF-kappa B pathway. Anat Sci Int 2017; 92: 330-337. [DOI:10.1007/s12565-016-0336-z]
15. Gholami M, Zendedel A, Khanipour khayat Z, Ghanad K, Nazari A, Pirhadi A. Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats. Biol Trace Elem Res 2015; 164: 205-211. [DOI:10.1007/s12011-014-0218-y]
16. Khaksary Mahabady M, Gholami MR, Najafzadeh Varzi H, Zendedel A, Doostizadeh M. Protective effect of quercetin on skeletal and neural tube teratogenicity induced by cyclophosphamide in rat fetuses. Vet Res Forum 2016; 7: 133-138.
17. Zendedel A, Gharibi Z, Anbari K, Abbaszadeh A, Khayat ZK, Khorramabadi RM, et al. Selenium ameliorate peripheral nerve ischemic-reperfusion injury via decreased TNF-α. Biol Trace Elem Res 2017; 176: 328-337. [DOI:10.1007/s12011-016-0836-7]
18. Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 2004; 8: 616-627. [DOI:10.1016/S1472-6483(10)61641-0]
19. Davies KJ. Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life 2000; 50: 279-289. [DOI:10.1080/15216540051081010]
20. Gholami M, Ahmadi SAY, Abaszadeh A, Khaki A. Protective effects of melatonin and ghrelin on spermatogenesis: A narrative review of the literature. Int J Reprod Biomed 2017; 15: 265-272. [DOI:10.29252/ijrm.15.5.265]
21. Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 2001; 74: 418-425. [DOI:10.1093/ajcn/74.4.418]
22. Aktas C, Erboga M, Fidanol Erboga Z, Bozdemir Donmez Y, Topcu B, Gurel A. Protective effects of Urtica dioica L. on experimental testicular ischaemia reperfusion injury in rats. Andrologia 2017; 49: 1-5. [DOI:10.1111/and.12636]
23. Milcan A, Arslan E, Bagdatoglu OT, Bagdatoglu C, Polat G, Kanik A, et al. The effect of alprostadil on ischemia-reperfusion injury of peripheral nerve in rats. Pharmacol Res 2004; 49: 67-72. [DOI:10.1016/j.phrs.2003.07.008]
24. Aitken RJ, Ryan AL, Baker MA, McLaughlin EA. Redox activity associated with the maturation and capacitation of mammalian spermatozoa. Free Rad Biol Med 2004; 36: 994-1010. [DOI:10.1016/j.freeradbiomed.2004.01.017]
25. Moghimian M, Soltani M, Abtahi H, Adabi J, Jajarmy N. Protective effect of tunica albuginea incision with tunica vaginalis flap coverage on tissue damage and oxidative stress following testicular torsion: Role of duration of ischemia. J Pediatr Urol 2016; 12: 390.e1-390.e6. [DOI:10.1016/j.jpurol.2016.06.002]
26. Ford WC. Regulation of sperm function by reactive oxygen species. Hum Reprod Update 2004; 10: 387-399. [DOI:10.1093/humupd/dmh034]
27. Beigi Boroujeni M, Shahrokhi SS, Birjandi M, Abbaszadeh A, Beyranvand F, Hamoleh S, et al. Effects of pomegranate peel extract on histopathology, testosterone levels and sperm of testicular torsion-detorsion induced in adult Wistar rats. J Complement Integr Med 2017; 14: 1-5. [DOI:10.1515/jcim-2017-0009]
28. Ahmadi S, Bashiri R, Ghadiri-Anari A, Nadjarzadeh A. Antioxidant supplements and semen parameters: An evidence based review. Int J Reprod Biomed 2016; 14: 729-236. [DOI:10.29252/ijrm.14.12.729]
29. Elmussareh M, Mahrous A, Kayes O. Antioxidant therapy for male subfertility: myth or evidence‐based? Trend Urol Men Health 2015; 6: 35-39. [DOI:10.1002/tre.439]
30. Kumar TR, Wang Y, Lu N, Matzuk MM. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat Genet 1997; 15: 201-204. [DOI:10.1038/ng0297-201]
31. Ramaswamy S, Weinbauer GF. Endocrine control of spermatogenesis: Role of FSH and LH/ testosterone. Spermatogenesis 2015; 4: e996025. [DOI:10.1080/21565562.2014.996025]
32. Dierich A, Sairam MR, Monaco L, Fimia GM, Gansmuller A, LeMeur M, et al. Impairing follicle-stimulating hormone (FSH) signaling in vivo: targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance. Proc Nati Acad Sci USA 1998; 95: 13612-13617. [DOI:10.1073/pnas.95.23.13612]
33. Abel MH, Wootton AN, Wilkins V, Huhtaniemi I, Knight PG, Charlton HM. The effect of a null mutation in the follicle-stimulating hormone receptor gene on mouse reproduction. Endocrinology 2000; 141: 1795-1803. [DOI:10.1210/endo.141.5.7456]
34. Allan CM, Haywood M, Swaraj S, Spaliviero J, Koch A, Jimenez M, et al. A novel transgenic model to characterize the specific effects of follicle-stimulating hormone on gonadal physiology in the absence of luteinizing hormone actions. Endocrinology 2001; 142: 2213-2220. [DOI:10.1210/endo.142.6.8092]
35. Zini A, Al-Hathal N. Antioxidant therapy in male infertility: fact or fiction? Asian J Androl 2011; 13: 374-381. [DOI:10.1038/aja.2010.182]
36. Saitoh Y, Yoshimoto T, Kato S, Miwa N. Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell. Exp Oncol 2015; 37: 94-99.
37. Cruz Diaz LA, Flores Miramontes MG, Chavez Hurtado P, Allen K, Gonzalez Avila M, Prado Montes de Oca E. Ascorbic acid, ultraviolet C rays, and glucose but not hyperthermia are elicitors of human beta-defensin 1 mRNA in normal keratinocytes. Biomed Res Int 2015; 2015: 714580. [DOI:10.1155/2015/714580]
38. Fukumura H, Sato M, Kezuka K, Sato I, Feng X, Okumura S, et al. Effect of ascorbic acid on reactive oxygen species production in chemotherapy and hyperthermia in prostate cancer cells. J Physiol Sci 2012; 62: 251-257. [DOI:10.1007/s12576-012-0204-0]
39. Aktas A, Cudi Tuncer M, Yildirim A, Nergiz Y, Akkus M. Protective effects of melatonin on testicular torsion and detorsion damage in Sprague-Dawley rats. Int J Morphol 2011; 29: 7-15. [DOI:10.4067/S0717-95022011000100001]
40. Aktoz T, Kanter M, Aktas C. Protective effects of quercetin on testicular torsion/detorsion‐induced ischaemia‐reperfusion injury in rats. Andrologia 2010; 42: 376-383. [DOI:10.1111/j.1439-0272.2010.01044.x]
41. Azarneoshan F, Khatam Saz S, Sadeghi H. [The effects of hydro alcoholic extract of Dorema Aucheri on blood concentration of gonadotropin and androgen hormones in adult male rats.] Armaghane-e-Danesh 2009; 14: 63-70. (in Persian)
42. Dokmeci D, Kanter M, Inan M, Aydogdu N, Basaran UN, Yalcin O, et al. Protective effects of ibuprofen on testicular torsion/ detorsion- induced ischemia/ reperfusion injury in rats. Arch Toxicol 2007; 81: 655-663. [DOI:10.1007/s00204-007-0189-2]
43. Ghasemiboroon M, Ghafourian Boroujerdnia M, Ahangarpoor A, Kooti W, Hasanzadeh Noohi Z, Noori Ahmad Abadi M. [The effect of hydro-alcoholic extract of Celery (Apium graveolens) leaves on serum level of testosterone, FSH and LH in male rats]. J Zanjan Univ Med Sci 2014; 22: 49-57. (in Persian)
44. Ghasemneghad R, Mohammadghasemi F, Faghani M, Bahadori M. [Protective effect of oxytocin on quantitative and qualitative spermatogenesis parameters in rat's testis under ischemia-reperfusion]. J Guilan Uni Med Sci 2015; 23: 53-62. (in Persian)
45. Mokhtari M, Jowhari H, Yazdanpour F. [Effects of hydro-alcoholic seed extract of Coriandrum sativum L. on pituitary-ovary hormones in rat]. Med Sci 2013; 22: 237-243. (in Persian)
46. Najafi G, Nejati V, Shalizar Jalali A, Zahmatkesh E. Protective role of royal jelly in oxymetholone-induced oxidative injury in mouse testis. Iran J Toxicol 2014; 8: 1073-1080.
47. Jahromi V, Azhdari S, Ghaedi Sh, Farzam M, jahromi H. Effect of hydroalcoholic extract of Citrus aurantifolia peel on serum level of testosterone, FSH, LH and testis tissue in adult male rats. Int J Biol Pharm All Sci 2013; 2:1-9.
48. Akondi BR, Challa SR, Akula A. Protective effects of rutin and naringin in testicular ischemia-reperfusion induced oxidative stress in rats. J Reprod Infertil 2011; 12: 209-211.

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