Volume 16, Issue 4 (4-2018)                   IJRM 2018, 16(4): 267-274 | Back to browse issues page

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Kalhori Z, Soleimani Mehranjani M, Azadbakht M, Shariaatzadeh M A. Ovary stereological features and serum biochemical factors following induction of polycystic ovary syndrome with testosterone enanthate in mice: An experimental study. IJRM. 2018; 16 (4) :267-274
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1065-en.html
1- Department of Biology, Faculty of Science, Arak University, Arak, Iran
2- Department of Biology, Faculty of Science, Arak University, Arak, Iran , m-Soleimani@araku.ac.ir
3- Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Abstract:   (88 Views)
Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder featured by insulin resistance and hyperandrogenism. Testosterone enanthate can induce PCOS in mice models.
Objective: We investigated the ovary stereological features along with the oxidative stress and inflammatory factors in mice following PCOS induction using testosterone enanthate.
Materials and Methods: Twelve female NMRI mice (3 wk old) were divided into 2 groups (n=6/each): Control and PCOS. PCOS was induced through daily injections of testosterone enanthate (1 mg/100g subcutaneous s.c for 5 wk). Finally, ovaries were studied stereologically. The serum levels of the follicle-stimulating hormone, luteinizing hormone, testosterone, interleukin-6, and tumor necrosis factor-α were measured using ELISA kit. Serum levels of Malondialdehyde and the antioxidant capacity were measured relatively using thiobarbituric acid and ferric reducing antioxidant power assay.
Results: The mean total volume of ovary and the mean volume of cortex (p<0.001), volume of oocyte in the preantral (p=0.011) and antral follicle (p=0.015), thickness of zona pellucida (p=0.016), the number of antral follicles (p=0.012), the serum levels of follicle-stimulating hormone (p<0.001) and the antioxidant capacity (p=0.020) reduced significantly in the PCOS group compared to the control. The number of primary (p=0.017) and preantral (p=0.006) follicles and the serum levels of testosterone (p<0.001), Luteinizing hormone (p=0.002), Malondialdehyde, Interleukin 6 and Tumor necrosis factor-α (p<0.001) showed a significant increase in the PCOS group compared to the control.
Conclusion: Testosterone enanthate induced PCOS causes stereological features in the ovary, increases the oxidative stress and inflammatory markers in mice.
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Type of Study: Original Article | Subject: Reproductive biology
Received: 2018/05/27 | Accepted: 2018/05/27 | Published: 2018/05/27

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