Volume 16, Issue 4 (April 2018)                   IJRM 2018, 16(4): 247-254 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tunçdemir M, Yenmiş G, Tombultürk K, Arkan H, Soydaş T, Burak Tek R, et al . NFKB1 rs28362491 and pre-miRNA-146a rs2910164 SNPs on E-Cadherin expression in case of idiopathic oligospermia: A case-control study. IJRM. 2018; 16 (4) :247-254
URL: http://journals.ssu.ac.ir/ijrmnew/article-1-1062-en.html
1- Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
2- Department of Urology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
3- Department of Medical Biology and, Genetics, Medical Faculty, Istanbul Aydin University, Istanbul, Turkey , kanigur@istanbul.edu.tr
Abstract:   (413 Views)
Background: A notable proportion of idiopathic male infertility cases is accompanied by oligozoospermia; and yet, the molecular mechanisms of fertilization problem underlying this defect are still unclear. Epithelial cadherin has been involved in several calcium-dependent cell-to-cell adhesion events; however, its participation in gamete interaction has also not been fully investigated.
Objective: The aim was to investigate the changes in the expression of E-cadherin, based on the frequency of Single nucleotide polymorphisms in Nuclear Factor Kappa-B 1 and pre-mir-146a in oligospermic men.
Materials and Methods: In this case-control study, semen and blood samples of 131 oligospermic men as the case group and 239 fertile healthy men as the control group were analyzed. Variants single nucleotide polymorphisms rs28362491 and rs2910164 were performed using polymerase chain reaction-restriction fragment length polymorphism method and E-cadherin expression were determined by immunoprecipitation studies.
Results: ins/ins genotype of rs28362491 was determined as a risk factor for idiopathic oligospermia by 1.73 times (p=0.0218), whereas no significant differences were found between the groups concerning pre-mir-146a rs2910164 polymorphism (p=0.2274 in case of GC genotype and p=0.9052 in case of GG genotype). Combined genotype analysis results did not show any notable differences between the multiple comparisons of 28362491-rs2910164 in oligospermic men and control groups. In addition, E-cadherin expression of oligospermic men with ins/ins genotype was significantly lower than patients with del/ins genotype (p=0.0221). E-cadherin expression level was low in oligospermic men with respect to the control group in presence of ins/ins genotype of NFKB1 gene.
Conclusion: These results suggest that ins allele prevents binding of surface proteins to spermatozoa, leading to a low affinity of sperm-oocyte interaction in oligospermic men.
Full-Text [PDF 527 kb]   (211 Downloads) |   |   Full-Text (HTML)  (57 Views)  
Type of Study: Original Article | Subject: Reproductive biology
Received: 2018/05/27 | Accepted: 2018/05/27 | Published: 2018/05/27

1. Guzick DS, Overstreet JW, Factor-Litvak P, Brazil CK, Nakajima ST, Coutifaris C, et al. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2001; 345: 1388-1393. [DOI:10.1056/NEJMoa003005]
2. Carlsen H, Alexander G, Austenaa L, Ebihara K, Blomhoff R. Molecular imaging of the transcription factor NF-kB, a primary regulator of stress response. Mutat Res 2004; 551: 199-211. [DOI:10.1016/j.mrfmmm.2004.02.024]
3. Yamamoto Y, Gaynor RB. IkappaB kinases: key regulators of the NF-kappaB pathway. Trends Biochem Sci 2004; 29: 72-79. [DOI:10.1016/j.tibs.2003.12.003]
4. Sultuybek GK, Yenmis G, Koc A. A General Sight about Linking PARP-1 and NFKB1 Variations to the Inflammatory Events. Interdiscip J Microinflammation 2014; 1: 1-9.
5. Karban AS, Okazaki T, Panhuysen CI, Gallegos T, Potter JJ, Bailey-Wilson JE, et al. Functional annotation of a novel NFKB1 promoter polymorphism that increases risk for ulcerative colitis. Hum Mol Genet 2004; 13: 35-45. [DOI:10.1093/hmg/ddh008]
6. Bianco B, Lerner TG, Trevisan CM, Cavalcanti V, Christofolini DM, Barbosa CP. The nuclear factor-kB functional promoter polymorphism is associated with endometriosis and infertility. Hum Immunol 2012; 73: 1190-1193. [DOI:10.1016/j.humimm.2012.08.008]
7. Solanas G, Porta-de-la-Riva M, Agustí C, Casagolda D, Sánchez-Aguilera F, Larriba MJ, et al. E-Cadherin controls beta-catenin and nf-kappa b transcriptional activity in mesenchymal gene expression. J Cell Sci 2008; 121: 2224-2234. [DOI:10.1242/jcs.021667]
8. Aberle H, Schwartz H, Kemler R. Cadherin-catenin complex: protein interactions and their implications for cadherin function. J Cell Biochem 1996; 61: 514-523. https://doi.org/10.1002/(SICI)1097-4644(19960616)61:4<514::AID-JCB4>3.0.CO;2-R [DOI:10.1002/(SICI)1097-4644(19960616)61:43.0.CO;2-R]
9. Hernandez Gifford JA, Hunzicker-Dunn ME, Nilson JH. Conditional deletion of beta-catenin mediated by Amhr2cre in mice causes female infertility. Biol Reprod 2009; 80: 1282-1292. [DOI:10.1095/biolreprod.108.072280]
10. Purohit S, Brahmaraju M, Palta A, Shukla S, Laloraya M, Kumar PG. Impaired E-Cadherin expression in human spermatozoa in a male factor infertility subset signifies e-cadherin-mediated adhesion mechanisms operative in sperm-oolemma interactions. Biochem Biophys Res Commun 2004; 316: 903-909. [DOI:10.1016/j.bbrc.2004.02.133]
11. Barad O, Meiri E, Avniel A, Aharonov R, Barzilai A, Bentwich I, et al. MicroRNA expression detected by oligonucleotide microarrays: system establishment and expression profiling in human tissues. Genome Res 2004; 14: 2486-2494. [DOI:10.1101/gr.2845604]
12. Taganov KD, Boldin MP, Chang KJ, Baltimore D. NF-κB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Proc Natl Acad Sci USA 2006; 103: 12481-12486. [DOI:10.1073/pnas.0605298103]
13. Rusca N, Monticelli S. MiR-146a in Immunity and Disease. Mol Biol Int 2011; 2011: 437301. [DOI:10.4061/2011/437301]
14. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th Ed. 2010.
15. Cayli S, Jakab A, Ovari L, Delpiano E, Celik-Ozenci C, Sakkas D, et al. Biochemical markers of sperm function: male fertility and sperm selection for ICSI. Reprod Biomed Online 2003; 7: 462-468. [DOI:10.1016/S1472-6483(10)61891-3]
16. Tunçdemir M, Ozturk M. The effects of ACE inhibitor and angiotensin receptor blocker on clusterin and apoptosis in the kidney tissue of streptozotocin-diabetic rats. J Mol Histol 2008; 39: 605-616. [DOI:10.1007/s10735-008-9201-2]
17. Andersson AM, Edvardsen K, Skakkebaek NE. Expression and localization of N- and E-Cadherin in the human testis and epididymis. Int J Androl 1994; 17: 174-180. [DOI:10.1111/j.1365-2605.1994.tb01239.x]
18. Caballeroa JN, Gervasib MG, Veigaa MF, Dalvitc GC, Perez-Martínezb S, Ceticac PD, et al. Epithelial cadherin is present in bovine oviduct epithelial cells and gametes, and is involved in fertilization-related events. Theriogenology 2014; 81: 1189-1206. [DOI:10.1016/j.theriogenology.2014.01.028]
19. Rufas O, Fisch B, Ziv S, Shalgi R. Expression of cadherin adhesion molecules on human gametes. Mol Hum Reprod 2000; 6: 163-169. [DOI:10.1093/molehr/6.2.163]
20. Elcin P, Burak Tek R, Koc A, Arkan H, Ozkara H, Kanigur-Sultuybek G. Effects of SNPs in nuclear factor kappa-B1, poly (ADP-ribose) polymerase-1 genes on E-Cadherin and fibronectin levels in case of male infertility. Androl Gynecol Curr Res 2015; 3: 1-7.
21. Delfino F, Walker WH. Stage-specific nuclear expression of nf-kappa b in mammalian testis. Mol Endocrinol 1998; 12: 1696-1707.
22. Pentikainen V, Suomalainen L, Erkkilä K, Martelin E, Parvinen M, Pentikainen MO, et al. Nuclear factor-kappa B activation in human testicular apoptosis. Am J Pathol 2002; 160: 205-218. [DOI:10.1016/S0002-9440(10)64364-7]
23. Ranganathan P, Kattal N, Moustafa MH, Sharma RK, Thomas Jr AJ, Agarwal A. Correlation of nuclear factor kappa B (NFKB) with sperm quality and clinical diagnoses in infertile men. Fertil Steril 2002; 78 (Suppl.): S95. [DOI:10.1016/S0015-0282(02)03631-2]
24. Lian J, Zhang X, Tian H, Liang N, Wang Y, Liang C, et al. Altered microRNA expression in patients with non-obstructive azoospermia. Reprod Biol Endocrinol 2009; 7: 13-25. [DOI:10.1186/1477-7827-7-13]
25. Ro S, Park C, Sanders KM, McCarrey JR, Yan W. Cloning and expression profiling of testis-expressed microRNAs. Dev Biol 2007; 311: 592-602. [DOI:10.1016/j.ydbio.2007.09.009]
26. Wang C, Yang C, Chen X, Yao B, Yang C, Zhu C, et al. Altered profile of seminal plasma microRNAs in the molecular diagnosis of male infertility. Clin Chem 2011; 57: 1722-1731. [DOI:10.1373/clinchem.2011.169714]
27. Hashemi M, Eskandari-Nasab E, Zakeri Z, Atabaki M, Bahari G, Jahantigh M, et al. Association of pre-miRNA-146a rs2910164 and pre miRNA-499 rs3746444 polymorphisms and susceptibility to rheumatoid arthritis. Mol Med Rep 2013; 7: 287-291. [DOI:10.3892/mmr.2012.1176]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2019 All Rights Reserved | International Journal of Reproductive BioMedicine

Designed & Developed by : Yektaweb